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Perfusion bioreactor operation is based on culture medium convection across a porous cell construct, thus providing a controllable, mechanically active environment for all seeded cells. It was shown that cultivation of engineered tissues in perfusion bioreactors improved the construct size, cellularity, and molecular composition. The bioreactors also made it possible to study effects of (1) mass transport of growth factors and nutrients, (2) hydrodynamic load conditions, and (3) physical stimulation, such as dynamic compression or cyclic stretch, on the developing tissue (Carrier et al, 2002; Radisic et al, 2008).